This chapter describes the acquisition and processing of a one-dimensional 13C NMR spectrum. The standard Bruker parameter set C13CPD, includes the pulse sequence zgpg30, shown in the figure below. The 13C channel consists of the recycling delay, a RF pulse, and the acquisition time during which the signal is recorded. The pulse angle is shown to be 30°. The two parameters, d1 and p1, correspond to the length of the recycle delay, and the length of the 90° RF pulse, respectively. The 1H channel consists of two decoupling pulses which can be power gated. The first pulse, an NOE build-up pulse during the recycle delay may be of lower power then the second pulse during the acquisition which is the true decoupling pulse. This can be useful to avoid RF heating on salty samples or probes where a higher decoupling power can be problematic.

The time intervals depicted in the pulse sequence diagrams are not drawn to scale. For example, d1 is typically a few seconds, while p1 is typically a few microseconds in length.

The sample to be used is 30 mg of Menthyl Antranilate in DMSO-d6.