CHEM 455 BIOCHEMISTRY LABORATORY
Announcements
Sample Exam
Lecture Materials
Lecture Notes
Spring 2014 Lecture schedule
1. Cloning Lecture
2. Protein Purification Lecture
3. Structure
4.
PyMol
(instruction for making movies included)
5. β-Lactamase
Lab Materials
Chem 455 Course Info
Spring 2014 Lab Schedule
Cloning
pH Buffer
PCR
Spectrophotometry
Oral Presentations
Guidelines
Lab 1:
Pipette Calibration
Lab 2:
DNA isolation, Restriction Digest, and Electrophoresis
Lab 3:
Polymerase Chain Reaction
Lab 4:
Gel Filtration Chromatography
Lab 5:
Ion Exchange Chromatography
Lab 6A:
Protein Concetration Determination
Lab 6B:
Protein Analysis via SDS Electrophoresis
Lab 7:
β-Lactamase Activity Assay
